DNA Polymerase I Large (Klenow) Fragment
from
New England Biolabs
Description
Klenow fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3´ -> 5´ exonuclease activity, but has lost 5´ ->3´ exonuclease activity. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5´ termini.
Specifications/Features
Unit Definition:
One unit is defined as the amount of enzyme required to convert 10 nmoles of dNTPs to an acid-insoluble form in 30 minutes at 37°C.
Comments:
DNA sequencing by the Sanger dideoxy method Fill-in of 3´ recessed ends Second-strand cDNA synthesis Random priming labeling. Second strand synthesis in mutagenesis protocols.