DNA Polymerase I Large (Klenow) Fragment
from
Invitrogen
Description
DNA Polymerase I, Large (Klenow) Fragment is a DNA polymerase that Polymerase I, but it contains the 3´ 5´ exodeoxyribonuclease activity.
Applications: Fill-in of 5´ overhangs (1). Synthesis of probes by random primers labeling method (2). Sequencing single- and double-stranded DNA (3). Site-directed mutagenesis.
Source: Purified from E. coli expressing the Klenow fragment on a plasmid.
Performance and Quality Testing: SDS-PAGE purity; single-stranded and double-stranded endodeoxyribonuclease and self-priming assays; performance evaluated in fill-in reaction.
Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a
template•primer.
Specifications/Features
Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using poly(dA-dT) as a templateprimer.
Comments:
18012039