EnzChek® Peptidase/Protease Assay Kit
from
Invitrogen
Description
The EnzChek® Peptidase/Protease Assay Kit (E33758) provides a FRET (fluorescence resonance energy transfer)-based method for the simple and accurate quantitation of a wide range of protease activities. The EnzChek® peptidase/protease substrate comprises a fluorophore and a quencher moiety separated by an amino acid sequence. Upon sequence cleavage by protease(s), the fluorophore separates from the quencher and is free to emit a detectable fluorescent signal (excitation and emission maxima of 502 and 528 nm, respectively). The magnitude of the resultant signal is proportional to the degree of substrate cleavage, and can therefore be used to quantitate the enzyme activity present. The assay is performed in a simple mix–incubate–read format, can be completed in 60 minutes or less, and is easily adaptable to accomodate diverse pH requirements.