BioModule™ Microarray direct labeling from Invitrogen

Description

BioModule™ Microarray Units includes major reagents required to purify your RNA, synthesize and label cDNA, then subsequently hybridize and wash your array. BioModule™ Microarray is available with SuperScript® Plus cDNA Labeling System optimized with Alexa Fluor® dyes to yield proven cDNA labeling results to support direct and indirect labeling methods. All BioModule™ Microarray units have been validated to ensure that they are compatible across your experiment.BioModule™ Microarray unit contains TRIzol® Reagent for RNA isolation and a PureLink™ Micro-to-Midi™ Total RNA Purification Kit for post-extraction clean up to ensure high-quality total RNA. The PureLink™ Kit is a one-hour procedure that requires no handling or disposal of organic waste. RNA binds to a silica membrane and impurities are removed by washing through vacuum filtration or centrifugation. High-purity total RNA is eluted into RNase-free water, ideal for immediate use in labeling.indirect labeling, mRNA or total RNA is reverse transcribed using SuperScript® III RT, incorporating amino-modified dUTP and dATP into the synthesized cDNA. The template RNA is then degraded by base hydrolysis, and the reaction is neutralized with acid. The amino-modified cDNA is then purified to remove unincorporated nucleotides, primers, and buffers. In the second step, the modified cDNA is coupled with the active form of Alexa Fluor® 555 or 647 fluorescent dye. The labeled cDNA is purified with a low-elution volume spin column to remove any unreacted dye. The resulting cDNA is ready for hybridization to microarrays.direct labeling, an anchored oligo(dT) anneals to the mRNA template. SuperScript® III Reverse Transcriptase (RT) extends from the priming site, incorporating dUTP labeled with Alexa Fluor® dye directly to produce labeled cDNA. Template RNA is degraded by base hydrolysis. Free nucleotides and other contaminants are removed using low-elution volume purification columns before hybridization to the microarray.Contents and Storage:BioModule™ Microarray with direct labeling includes TRIzol® Reagent, PureLink™ Micro-to-Midi™ Total RNA Purification Kit, SuperScript® Plus Direct cDNA Labeling System for DNA Microarrays with Alexa Fluor® 555- and 647-aha-dUTP dyes. Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C.™ Microarray with indirect labeling includes PureLink™ Micro-to-Midi™ Total RNA Purification Kit, and SuperScript® Plus Indirect cDNA Labeling System for DNA Microarrays. Store purification columns, loading buffer, and wash buffer at room temperature. Store all other components at -20°C.addition, each BioModule™ Microarray unit provides several key reagents necessary for the post-labeling steps, including hybridization, and washing.* Visit www.invitrogen.com for the list of components within each BioModule™ Application Unit. BioModule™ Microarray units are guaranteed for 60 days from the date of purchase

• High-Purity RNA, Superior-Quality cDNA, Fast and Efficient Protocol for Improved Array Labeling