Telomere PNA Kit/FITC for Flow Cytometry
from
Dako
Description
Telomere PNA Kit/FITC for Flow Cytometry provides a convenient method for measuring telomeric sequences in vertebrate interphase hematopoietic cells. The kit contains reagents for 20 duplicate tests (40 single tests). In addition to the fluorescein-conjugated peptide nucleic acid (PNA) probe in hybridization solution, the kit contains hybridization solution without probe for correction of cell autofluorescence, wash solution for post-hybridization washes and DNA-staining solution for identification of G0/1-cells. The kit has been designed so that post-hybridization washes are kept to a minimum and formamide washes are avoided. In a mixture of sample cells (provided by the user) and control cells (provided by the user), the sample DNA is denatured at 82 °C for 10 minutes in an Eppendorf tube in the presence of hybridization solution with or without fluorescein-conjugated PNA telomere probe. Then, hybridization takes place in the dark at room temperature overnight. The hybridization is followed by 2 washes in wash solution at 40 °C for 10 minutes each. Finally the cells are resuspended in DNA-staining solution and stored in the dark at 2-8 °C for 2-3 hours before analysis by flow cytometry. The specific fluorescence from telomere staining will be observed in FL1, and fluorescence from DNA staining will be observed in FL3. Compared with the traditional telomere restriction fragment (TRF) method, a major advantage of the Dako Telomere PNA Kit/FITC assay is that it does not suffer from the interaction of subtelomere sequences.
EU regulatory status: RUO
US regulatory status: RUO