Standard Protein Kinase Substrate (PKS) Peptide Microarray
from
LC Sciences
Description
Microarrays designed for proteomic scale kinase profiling, quantitative measurement of kinase kinetic activities, and drug discovery research built on the flexible and powerful µParaflo® microfluidic on-chip synthesis platform. These microarrays are available as part of our comprehensive Protein Kinase Substrate - Peptide Microarray Service.
Flexible Probe Content
Our standard content PKS – peptide microarray includes a comprehensive list of kinases and their related experimentally verified Serine, Threonine, and Tyrosine phosphorylation sites compiled from the Phospho.ELM database. Customization of standard arrays is permitted.
High-Throughput Format
Traditional kinase assays focus on a single to a few peptide or protein substrates. Profiling kinases on a peptide microarray offers the opportunity to study thousands of specific substrates in a single experiment. One experiment using our protein kinase substrate (PKS) - peptide microarray is equivalent to 40 or more experiments using a 96-well plate.
Innovative µParaflo® Microarray Platform
These are not spotted arrays. The µParaflo® microfluidic technology enables on-chip synthesis ensuring high probe quality, tight process control, and complete content flexibility. In addition, the microfluidic technology produces a uniform distribution of the sample solutions on the array and enhances binding reactions and stringency wash processes.
Quantitative Results
Well designed positive and negative controls as well as a universal highly phosphate-specific fluorescent dye staining reagent ensure quantitative results. There is no need for radioisotope labeling and potential signal bias due to antibody sequence specificity is eliminated. Test sequence signals can be directly compared to phosphopeptide signals (considered to be 100% of phosphorylation reading).