α Integrin-mediated Cell Adhesion Array Kit, fluorimetric
from
EMD Millipore
Description
α Integrin-mediated Cell Adhesion Array Kit, fluorimetric Cell Adhesion. Introduction Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. A family of cell surface receptors, the integrins, mediates cell attachment to proteins of the extracellular matrix. Integrins are heterodimer molecules containing an alpha and beta transmembrane glycoprotein subunit that are non-covalently bound together (1-2). Historically, antibodies have been used to determine the integrin profiles of cells by immunoprecipitation, immunofluorescence, immunoblotting, and flow cytometry. These methods are time-consuming, laborious or require sophisticated equipment. The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Alpha Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of alpha integrin surface expression. In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including: · Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM534) · Alpha/Beta Integrin-Mediated Cell Adhesion Combo Array Kit (Fluorimetric) (ECM535) · Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530) · Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531) · Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532) · QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510) · QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512) · QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515) · QCM™ 96-well Cell Invasion Assay (ECM555) · QCM™ 96-well Collagen-Based Cell Invasion Assay (ECM 556) · 24-well Insert Cell Migration and Invasion assay systems · CytoMatrix™ Cell Adhesion strips (ECM protein coated) · QuantiMatrix™ ECM protein ELISA Kits Test Principle The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array Kit uses mouse monoclonal antibodies generated against human alpha integrins/subunits (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, and alphavbeta3), that are immobilized onto a goat anti-mouse antibody coated microtiter plate. The plate is then used to capture cells expressing these integrins on their cell surface; goat anti-mouse antibody coated wells are provided as a negative assay control. Each strip (8 wells total) consists of 7 integrin antibody coated wells and one negative control well (see plate layout on page 3). Experimental cells are seeded onto the coated substrate and incubated. Subsequently, unbound cells are washed away, and the adherent cells are lysed and detected by the patented CyQuant GR dye (Molecular Probes) (3-4). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (5). Relative cell attachment is determined using a fluorescence plate reader. Additional information about the monoclonals provided in the kit: Monoclonal / Known Species Reactivity / Clone Number Mouse anti-Alpha1 [CD49a] / Hu / FB12 Mouse anti-Alpha2 [CD49b] / Hu / P1E6 Mouse anti-Alpha3 [CD49c] / Hu / P1B5 Mouse anti-Alpha4 [CD49d] / Hu, Prim / P1H4 Mouse anti-Alpha5 [CD49e] / Hu / P1D6 Mouse anti-AlphaV [CD51] / Hu / P3G8 Mouse anti-AlphaV/Beta3 [CD51/CD61] / Hu, Bov, Por, Av, Can, Chk, Mky, Rb / LM609
Specifications/Features
Alpha Integrin Array Plate: (PN: 90599) One 96-well plate with 12 strips. Each strip contains one well each of the mouse anti-alpha integrin monoclonal antibodies: (alpha1, alpha2, alpha3, alpha4, alpha5, alphaV, alphavbeta3), and one goat anti-mouse negative well. See the plate layout on insert data sheet.;4X Cell Lysis Buffer: (Part No. 90130) One bottle -- 16 mL;CyQuant GR Dye®: (Part No. 90132) One vial -- 75 µL;Assay Buffer: (Part No. 90601) One bottle - 100 mL