PureYield Plasmid Maxiprep System from Promega

Product Specs
ItemPureYield Plasmid Maxiprep System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA2392
Timeapproximately 60 minutes
Volume250ml bacterial culture
Yield1 mg from 250 ml bacterial culture
Methodsilica-membrane column
Quantity10 preps
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification
Sample TypeBacterial culture

Specifications/Features

Phenol / Chloroform:
no

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Challenges and advances for huntingtin detection in cerebrospinal fluid: in support of relative quantificationBiomark ResApril 21, 2025Rachel J. Harding, Yuanyun Xie, Nicholas S. Caron, Hailey Findlay-Black, Caroline Lyu, Nalini Potluri, Renu Chandrasekaran, Michael R. Hayden, Blair R. Leavitt, Douglas R. Langbehn, et al.Sanger sequencing, then cultures were grown for large-scale DNA purification (Promega Maxiprep Kit, cat# A2392). HEK293 cells were plated at 3 × 105 cells per well in a 6-well plate and grown overnight to 80% confluence,

(2) Force-activated zyxin assemblies coordinate actin nucleation and crosslinking to orchestrate stress fiber repairCurrent BiologyFebruary 24, 2025Donovan Y.Z. Phua, Xiaoyu Sun, Gregory M. Alushinassays QIAprep spin miniprep kit,Qiagen,Cat# 27106 PureYield plasmid maxiprep system,Promega,Cat# A2392 SE Cell Line 4D-Nucleofector X Kit L,Lonza,Cat# V4XC-1024 Deposited data,Deposited data,Deposited

(3) Antibody-antibiotic conjugate targeted therapy for orthopedic implant-associated intracellular S. aureus infectionsJournal of Advanced ResearchNovember 1, 2024Leilei Qin, Ning Hu, Yanhao Zhang, Jianye Yang, Liqun Zhao, Xiaokai Zhang, Yun Yang, Jinyong Zhang, Yinshuang Zou, Keyu Wei, et al.peripheral blood mononuclear cells (PBMC) based on a single B cell technique[45]. PureYield™ Plasmid Maxiprep System (Promega, A2392) was used to extract heavy and light Plasmid DNA of 0662. The plasmid was transfected into HEK293F suspension

(4) Functional pathogenicity of ESRRB variant of uncertain significance contributes to hearing loss (DFNB35)Scientific ReportsSeptember 11, 2024Won Hoon Choi, Yeijean Cho, Ju Hyuen Cha, Dae Hee Lee, Jong Gwan Jeong, Sung Ho Jung, Jae-Jin Song, Jun Ho Lee, Sang-Yeon LeeDNA sequencing. The extracted plasmid DNA was then purified and concentrated using the plasmid maxi kit (A2392, Promega) as per the provided instructions, and subsequently stored at a temperature of − 20 °C for

(5) Protocol for the development of mRNA lipid nanoparticle vaccines and analysis of immunization efficiency in miceSTAR protocolsJune 21, 2024Karekar N, Reid Cahn A, Morla-Folch J, Saffon A, Ward RW, Ananthanarayanan A, Teunissen AJP, Bhardwaj N, Vabret N.NWW0041 Sodium pyruvate (100 mM),Life Technologies,11360-070 PureYield plasmid maxiprep system,Promega,#A2392 Monarch RNA cleanup kit,New England Biolabs,#T2050L DNA clean and concentrate,Zymo research,#D4033 MEM

(6) Effect of various types of extracellular DNA on V. hyugaensis biofilm formationmSphereAugust 24, 2023Carmen Gu Liu, Anthony W. Maressowith equal volumes of the lysis buffer from the PureYield Plasmid Maxiprep Kit (Promega, catalog no. A2392). This resuspension was centrifuged (5,000 rpm, 15 min, 4°C), and the supernatant was recovered. Then,

(7) Relating individual cell division events to single-cell ERK and Akt activity time coursesScientific ReportsOctober 27, 2022Alan D. Stern, Gregory R. Smith, Luis C. Santos, Deepraj Sarmah, Xiang Zhang, Xiaoming Lu, Federico Iuricich, Gaurav Pandey, Ravi Iyengar, Marc R. BirtwistleTransformations with the correct sequence were maxiprepped with PureYield Plasmid Maxiprep System (Promega #A2392) and used for lentiviral production. We exchanged the antibiotic selectable marker on the lentiviral

(8) Depletion of skeletal muscle satellite cells attenuates pathology in muscular dystrophyNature communicationsMay 26, 2022Boyer JG, Huo J, Han S, Havens JR, Prasad V, Lin BL, Kass DA, Song T, Sadayappan S, Khairallah RJ, et al.Escherichia coli (ThermoFisher, # 18265017), purified using the PureYield Plasmid Maxiprep System (Promega, #A2392) and were sent to our in-house viral core for packaging and large scale purification. A third virus

(9) Mechanosensing through Direct Binding of Tensed F-Actin by LIM DomainsDevelopmental CellNovember 1, 2020Xiaoyu Sun et al.purified by QIAprep spin miniprep kit (Qiagen, 27106) or PureYield plasmid maxiprep system (Promega, A2392) before transfection. Cell maintenance and mechanobiology Transient transfection MEFs were harvested

(10) Three-dimensional growth of breast cancer cells potentiates the anti-tumor effects of unacylated ghrelin and AZP-531eLifeJuly 15, 2020Au CC, Furness JB, Britt K, Oshchepkova S, Ladumor H, Soo KY, Callaghan B, Gerard C, Inghirami G, Mittal V, et al.,PureYield Plasmid Miniprep System,Promega,Cat# A1223, Commercial assay or kit,PureYield Plasmid Maxiprep System,Promega,Cat# A2392, "Chemical compound, drug","Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)",List Biological

(11) FAMIN Is a Multifunctional Purine Enzyme Enabling the Purine Nucleotide CycleCellJanuary 23, 2020Cader MZ, de Almeida Rodrigues RP, West JA, Sewell GW, Md-Ibrahim MN, Reikine S, Sirago G, Unger LW, Iglesias-Romero AB, Ramshorn K, et al.flow Strep-Tactin XT Superflow,IBA GMBH,Cat#2-4012-001 PureYield Plasmid Maxiprep System,Promega,Cat#A2392 cOmplete Mini EDTA-free Protease Inhibitor Cocktail,Sigma-Aldrich,Cat#11836170001 Ni-NTA Superflow

(12) Monoclonal Antibody 2C6 Targets a Cross-Clade Conformational Epitope in gp41 with Highly Active Antibody-Dependent Cell CytotoxicityJournal of VirologySeptember 1, 2019Hakimuddin Sojar, Sarah Baron, Jonathan T. Sullivan, Meghan Garrett, Marlies M. van Haaren, Jonathon Hoffman, Julie Overbaugh, Benjamin J. Doranz, Mark D. Hicarpaired heavy- and light-chain Ab plasmid DNA were ampliﬁed using the PureYield plasmid maxiprep system (A2392; Promega) by following the manufacturer’s instructions. Plasmids were then cotransfected into Free Style

(13) Inhibition of androgen receptor promotes CXC-chemokine receptor 7-mediated prostate cancer cell survivalScientific ReportsDecember 1, 2017James J. Hoy, Georgios Kallifatidis, Diandra K. Smith, Bal L. Lokeshwar(Promega; Madison, WI; E6751), amplified, and isolated with PureYield Plasmid Maxiprep System (Promega; A2392). Cells were transfected with the plasmid using Lipofectamine 2000 (Thermo Fisher; 11668027), and selected

(14) A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinningNature ProtocolsJune 11, 2009Florence Teulé, Alyssa R Cooper, William A Furin, Daniela Bittencourt, Elibio L Rech, Amanda Brooks, Randolph V LewisQIAprep Spin Miniprep Kit (Qiagen, cat. no. 27104) PureYield Plasmid Maxiprep System (Promega, cat. no. A2392) ABI PRISM BigDye terminator Cycle Sequencing Ready Reaction Kit (Perkin Elmer Applied Biosystems,

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11/21/2022High Yield of Transfection-Quality Plasmid DNA Using this Kit Wei Yang SitThe kit purifies up to 1mg of transfection-quality plasmid DNA from 250ml bacterial cultures in under an hour. The kit also include a unique Endotoxin Removal Wash designed to remove substantial ... Read Review