Description
Mononuclear cells are first obtained by light density separation of freshly collected samples. Lineage-selected cells are then isolated from the MNC population by negative selection using immunomagnetic cell separation procedures, and the purity of isolated cells is greater than 80% by flow cytometric analysis. Cells are cryopreserved in a 1.8 ml solution of 50% IMDM, 40% FBS, 10% DMSO (0.2 µm filtered).