Description
Antigen: Synthetic peptide (YKASpSPAESS) corresponding to amino acids 727-736 of human IRS 2 at the phosphorylated site of Serine 731. This synthetic peptide also correlates to amino acids 129-138 of mouse IRS 2 at the phosphorylation sites of Serine 133 and to amino acids 229-238 of rat IRS 2 at the serinephosphorylation site 233.
Cross-Reactivity: Cross-reacts with Human. Not yet tested in other species.
Applications: Immunoprecipitation, Western Blotting and ELISAThis antibody detects a band of approximately 200 kDa in cell lysate derived from mouse serum-treated fibroblasts, which corresponds to the predicted molecular weight of phospho IRS 2. Samples were boiled prior to subject 7.5% SDS-polyacrylamide gel electrophoresis.