Allele Frequency Analysis
from
Sequenom, Inc.
Description
Sequenom’s powerful MassARRAY system coupled with the Homogenous MassEXTEND assay provides rapid, accurate SNP validation using a sample-pooling technique — allele frequency — that rapidly screens and confirms the presence of a SNP and its allelic frequency in patient populations.
Conventional technologies typically analyze each SNP in each individual of the population in question, and individual results are then consolidated to yield the overall SNP allele-frequency distribution.
MassARRAY technology is able to determine SNP allele frequencies with high precision in pooled samples, thereby replacing hundreds of individual measurements with one consolidated analysis. With MassARRAY, you have the capability to pre-evaluate SNPs, which means that only SNPs that prove valuable in the pool-to-pool comparison are included in individual genotyping projects, significantly reducing the total workload for individual genotyping.
- Recommend screening 300-500 individuals per patient pool for greatest statistical relevance
- As little as 25 ng DNA per patient pool (83 pg/individual patient)
- Sensitivity to detect alleles frequencies as low as 3% on average
- Can scan nearly 200,000 individuals in an hour (384 reactions per hour, 500 individuals per reaction)
- Heterozygote skew analysis available