Cholesterol Colorimetric / Fluorometric Assay Kit
from
EMD Millipore
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Product
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Cholesterol Colorimetric / Fluorometric Assay Kit
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Detection
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Request Info
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Form
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96-well plate
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Target
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Cholesterol
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Catalog Number
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428901-1KIT
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Method
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Colorimetric / Fluorometric
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Original Item Name
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Cholesterol/Cholesteryl Ester Quantitation Kit
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Quantity
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100 Tests
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Range
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0.02-10 µg/well
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Sample Type
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Cells, tissues, serum
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Sensitivity
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4-10 fold more sensitive than colorimetric assay
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Description
100 Tests. PROTECT FROM LIGHT. A sensitive colorimetric or fluorometric assay for the quantitative measurement of cholesterol, cholesteryl ester, or both, by either methods. Measurement by spectrophotometry at OD =570 nm or fluorometry at Ex/Em =535/587 nm. This assay can measure cholesterol itself, total cholesterol (cholesterol + cholesterol ester) by adding cholesterol esterase to the reaction, or only cholesteryl ester by subtracting the value of cholesterol from the total value of cholesterol plus cholesteryl esters. The fluorometric assay can detect 0.02-1 µg/well and is 4-10 fold more sensitive than colorimetric assay. Kit contains sufficient reagents for 100 tests.
References
Vivanco, I., et al. 2007. Cancer Cell 11, 555.Szczepankiewicz, B. G., et al. 2006. J. Med. Chem. 49, 3563.
Specifications/Features
Kit Contents:
A sensitive colorimetric or fluorometric assay for the quantitative measurement of cholesterol, cholesteryl ester, or both, by either methods. Measurement by spectrophotometry at OD =570 nm or fluorometry at Ex/Em =535/587 nm. This assay can measure cholesterol itself, total cholesterol (cholesterol + cholesterol ester) by adding cholesterol esterase to the reaction, or only cholesteryl ester by subtracting the value of cholesterol from the total value of cholesterol plus cholesteryl esters. The fluorometric assay can detect 0.02-1 µg/well and is 4-10 fold more sensitive than colorimetric assay. Kit contains sufficient reagents for 100 tests.