pKillerRed-dMito vector
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Evrogen JSC
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Product
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pKillerRed-dMito vector
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Company
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Evrogen JSC
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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KillerRed can be used for directional killing of bacterial or eukaryotic cells and for precise protein photoinactivation in vivo. KillerRed or KillerRed-fusion protein can be expressed in cells or cell organelles of interest from KillerRed expression vector. If required, stable KillerRed transformants can be selected using G418.
KillerRed-mediated ROS generation should be induced by green light irradiation at 540-580 nm. Intensity of green light and irradiation time must be individually determined for particular biological system and instrumentation.
KillerRed-mediated ROS production is accompanied by profound KillerRed photobleaching. The resulting cell events (cell fate after irradiation, effect on protein localization) can be monitored using another fluorescent reporter, for example a green fluorescent protein. Vector sets comprising KillerRed and TurboGFP expression vectors are available from Evrogen.
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Conjugate/Tag/Label
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KillerRed is a novel non-aggregating red fluorescent protein capable of reactive oxygen species (ROS) generation upon green light irradiation.
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Host
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Mammalian
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Origin of Replication
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SV40 origin of replication
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Promoter
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PCMV IE 1-589
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Resistance
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Kan/Neo
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Type
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Plasmid
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Vector Name
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pKillerRed-dMito vector
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Catalog Number
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FP964
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Quantity
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20 µg
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Description
KillerRed is a red fluorescent protein capable of generation of reactive oxygen species (ROS) upon green light irradiation (Bulina et al., Nat Biotechnol. 2006, 24(1): 95-99). Unlike chemical photosensitizers, KillerRed can be directly expressed by target cells, both individually and in fusion with a target protein. It shows no cell toxic effects before light activation. Upon green light irradiation KillerRed generates ROS that damage the neighboring molecules. This novel tool allows precise inactivation of selected proteins in chromophore-assisted light inactivation (CALI) and high-specific light-induced cell killing. pKillerRed-dMito vector is appropriately used for the expression of mitochondria-targeted KillerRed in mammalian cells for precise light-induced cell killing.
Specifications/Features
Kit Contents:
20 µg
Vector Size:
4.85 kb
Features:
Genetically encoded photosensitizers