Description
This is an all purpose strain used for protein expression from vectors containing E. coli promoters. It is protease deficient and has a high level of protein expression. This strain lacks a T7 polymerase gene and can be used for non-T7 RNA polymerase protein expression systems. For T7 promoter driven protein expression, this strain requires infection with Lambda CE6 bacteriophage. Transformation efficiency >1 X 107 cfu/μg of pUC19