Akt1 / PKBa Kinase Fluorescence superquenching Assay Kit, TruLight™
from
EMD Millipore
Description
A sensitive and high speed homogeneous assay for detection of enzyme activity and for screening of Akt1/PKBα (Protein Kinase B alpha) inhibitors in a high-throughput screening (HTS) format. This kit is based on a fluorescence superquenching technology that does not require antibodies or radioactive label.
reference:
Rininsland, F., et al. 2004.
Proc. Natl. Acad. Sci. USA 101, 15295;Xia, W., et al. 2004.
Assay Drug Develop. Technol. 2, 183;Jones, R.M., et al. 2001.
Proc. Natl. Acad. Sci. 98, 14769;Chen, L., et al. 1999.
Proc. Natl. Acad. Sci. 96, 12287;Zhang, J.H., et al. 1999.
J. Biomol. Screen. 4, 67;Edelman, A.M., et al. 1987.
Annu. Rev. Biochem. 56, 567.
References
Rininsland, F., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 15295.Xia, W., et al. 2004. Assay Drug Develop. Technol. 2, 183.Jones, R.M., et al. 2001. Proc. Natl. Acad. Sci. 98, 14769.Chen, L., et al. 1999. Proc. Natl. Acad. Sci. 96, 12287.Zhang, J.H., et al. 1999. J. Biomol. Screen. 4, 67.Edelman, A.M., et al. 1987. Annu. Rev. Biochem. 56, 567.
Specifications/Features
Kit Contents:
A sensitive and high speed homogeneous assay for detection of enzyme activity and for screening of Akt1/PKBα (Protein Kinase B alpha) inhibitors in a high-throughput screening (HTS) format. This kit is based on a fluorescence superquenching technology that does not require antibodies or radioactive label.