Pepsin , Agarose Conjugated/Tagged
from
Thermo Scientific Pierce Protein Research Products
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Product
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Pepsin , Agarose Conjugated/Tagged
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Company
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Thermo Scientific Pierce Protein Research Products
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Antibody Fragment Applications:
Immunohistochemistry
Immunoassays, including in vitro diagnostic assays
Radioimmunolocalization for tumor detection and radiotherapy
Immunotargeting through the use of fragment conjugates as immunotoxins
Crystallographic study of antibody-binding sites
Study of Fc binding proteins and effector functions
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Conjugate/Tag/Label
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Agarose
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Molecule Name
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Pepsin
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Original Item Name
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PEPSIN-AGAROSE
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Quantity
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5 ml
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Species
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Request Info
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Catalog Number
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20343
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Description
Pierce has developed a complete kit to digest human or mouse IgG molecules into Fab fragments and Fc fragments by using immobilized papain enzyme. After digestion, the fragments are purified on an immobilized Protein A column provided in the kit. Detailed instructions allow for flexibility in the protocol for hard to digest antibodies.
Antibody Fragment Applications:
- Immunohistochemistry
- Immunoassays, including in vitro diagnostic assays
- Radioimmunolocalization for tumor detection and radiotherapy
- Immunotargeting through the use of fragment conjugates as immunotoxins
- Crystallographic study of antibody-binding sites
- Study of Fc binding proteins and effector functions
Features
Fab Fragments:
Will not be affected by Fc receptors on cells such as macrophages, B-cells, T-cells, neutrophils and mast cells Will not precipitate antigen Easier to make and purify than F(ab')2 fragments More rapid clearance of radiolabeled fragments from normal tissue than whole IgG conjugates Reduced immunogenicity (as a result of Fc region absence), minimizes human anti-mouse immunoglobulin (HAMA) response Fragments less susceptible to phagocytosis Fc Fragments:
Useful for studying effector functions of IgG without interference from antigen binding sites Fc fragments can be used as blocking agents for histochemical staining The easiest, most convenient way to generate Fab fragments from IgG.