T4 Polynucleotide Kinase
from
Promega
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Product
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T4 Polynucleotide Kinase
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Company
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Promega
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Price
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More Information
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View Company Product Page
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Activity
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One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [γ-32P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 5mM DTT, 0.1mM [γ-32P] ATP and 0.5μg/μl 5´-OH polynucleotide end concentration.
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Applications
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May be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.
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Ask A Scientist
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Conjugate/Tag/Label
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Unconjugated
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Form
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Concentration: 5–10u/μl
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Molecule Name
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Polynucleotide Kinase
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Original Item Name
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T4 Polynucleotide Kinase
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Source/Expression System
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Recombinant, E.coli
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Species
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T4
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Catalog Number
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M4103
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Quantity
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1,000 U
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Description
T4 Polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.
PNKP
(Polynucleotide kinase 3 prime phosphatase)