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T4 Polynucleotide Kinase from Promega
Product T4 Polynucleotide Kinase
Company Promega
Price
More Information
More Info
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Catalog Number M4101
Quantity 100 U
Activity One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [γ-32P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 5mM DTT, 0.1mM [γ-32P] ATP and 0.5μg/μl 5´-OH polynucleotide end concentration.
Applications May be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.
Species T4
Conjugate/Tag Unconjugated
Molecule Name Polynucleotide Kinase
Source/Expression System Recombinant, E.coli
Form/Grade/Purity Concentration: 5–10u/μl

Description

T4 Polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

Journal Citations

Jenner et al., Kaposi's Sarcoma-Associated Herpesvirus Latent and Lytic Gene Expression as Revealed by DNA Arrays. Journal of Virology, 75:891-902.

Jordà et al., Upregulation of MMP-9 in MDCK epithelial cell line in response to expression of the Snail transcription factor. Journal of Cell Science, 118:3371-3385.

Arnvig et al., A high-affinity interaction between NusA and the rrn nut site in Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences, 101:8325-8330.

View all citations for T4 Polynucleotide Kinase from Promega

Gene-Specific Product Directory

PNKP (Polynucleotide kinase 3 prime phosphatase)

Promega Contact Information

Promega
2800 Woods Hollow Rd.
Madison, WI 53711-5399 USA

Customer Service: 800-356-9526

Tech Support: 1-800-356-9526

Fax Number: 800-356-1970

Web Site: http://www.promega.com

Note: Biocompare disclaims any information on this site. Price information is approximate list price and actual prices may vary.

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