Dynabeads® M-270 Streptavidin
from
Invitrogen Dynal AS
Description
Streptavidin-coupled Dynabeads® is the gold standard for isolation and
handling of biotinylated nucleic acids, antibodies or other biotinylated ligands
and targets. The very high binding affinity of the streptavidin-biotin
interaction (Kd=10^-15) is utilized in a vast number of applications.
These uniform and superparamagnetic beads are 2.8 µm in diameter, with a
monolayer – not a multilayer - of recombinant streptavidin covalently coupled to
the surface. This leaves the vast majority of the biotin binding sites
sterically available for binding, not only of free biotin, but also for binding
of biotinylated ligands/targets. They are hydrophilic, negatively charged and
show rapid liquid-phase reaction kinetics. Their specific and defined surface
allow for efficient capture, separation and downstream handling. The
streptavidin monolayer ensures negligible leakage, and the lack of excess
adsorbed streptavidin ensures batch consistency and reproducibility of your
results.
Benefits and Features:
- Direct and fast isolation of any biotinylated molecule
- Flexible protocols with gentle and efficient liquid-phase reaction kinetics
- Very low non-specific binding of nucleotides and nucleic acids
- Very low aggregation in high-salt hybridization buffers
- Well suited for nucleic acid applications with extreme demands
- Low non-specific binding of small and negatively charged proteins,
- Production follows a validated process in compliance with cGMP for medical
device
- Well suited for automated protocols
Applications: Over the past 15 years, Streptavidin-coupled Dynabeads® have been
used and cited for a very wide variety of applications. Examples include
direct/indirect isolation and downstream handling of nucleic acids,
proteins/peptides and other target molecules. Ideal for sequence specific
DNA/RNA capture in nucleic acid based diagnostics, specifically with samples
with a high chaotropic salt concentration, immunoassays involving small
biotinylated antigens and applications that are not compatible with BSA (these
beads are not blocked with BSA). Easily adaptated to automated processes. Did
you know that Dynabeads® are employed on more than 25,000 routine IVD
instruments worldwide?
Binding capacity: