HIS-Select™ Spin Columns
from
Sigma-Aldrich
Description
Pre-packed and ready-to-use HIS-Select Spin Columns allow for fast and consistent purification of small-scale crude cell extracts containing histidine-tagged proteins. The spin columns contain a matrix of silica particles and uses the HIS-Select patented non-charged, hydrophilic nickel chelating linkage chemistry. HIS-Select Spin Columns are highly selective for histidine-tagged proteins and exhibit very low non-specific binding of other proteins. These convenient Spin Columns allow for fast, one-step purification for the isolation of at least 150 µg of histidine-tagged protein in less than 15 minutes. Features and Benefits:
- High Selectivity for higher purity
- Convenient and Ready-to-Use
- Consistent protein binding each use
- Compatible with CelLytic™ cell lysis reagents
Patent/trademark/license (electronic): Covered by US Patent No 6,623,655, licensed under U.S. Patent No. 4,569,794 and are sold for research use only. Commercial use requires additional licenses. General description: Kit contains 3 collection tubes for every spin column.