SensoLyte® Red Protease Assay Kit *Fluorimetric*
from
AnaSpec
Description
The SensoLyte® Red Protease Assay Kit is widely used for continuous detection of generic protease activities. The kit uses a casein derivative that is heavily labeled with a rhodamine derivative, resulting in almost total quenching of the conjugates fluorescence. Compared to the SensoLyte® Green Protease Assay Kit, this kit contains a red fluorescent casein derivative. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides (Ex/Em=546/575 nm). The increase in fluorescence intensity is directly proportional to protease activity. The SensoLyte® protease assay kits do not require any separation steps and can be used to continuously measure the kinetics of a variety of exopeptidases and endopeptidases. The kit includes: · Fluorescently labeled casein with high ratio of dye/protein · Trypsin (as positive control) · Assay buffer · A detailed protocol
Specifications/Features
Proteases Detected:
Exopeptidases and endopeptidases.