RNAqueous®-Micro Kit 50 purifications
from
Ambion
Description
The Ambion® RNAqueous-Micro Kit is used for phenol-free
total RNA isolation from micro-sized samples such as 10–500,000 cultured
cells, as few as 10 laser capture microdissected cells, or up to 10 mg tissue
using a guanidinium-based lysis⁄;denaturant and glass fiber filter separation
technology. DNA-free™ DNA removal
reagents are included in the kit, making the isolated RNA especially suitable
for RT-PCR.
•Increased sensitivity in downstream assays
• Isolates high quality RNA from micro-scale samples
• Includes reagents⁄protocol for RNA recovery from LCM
samples
The RNAqueous-Micro Kit is optimized for the purification
of total RNA from micro-sized samples (10–500,000 cultured cells, as few
as 10 microdissected cells, or up to 10 mg tissue). Samples are solubilized in
a powerful chaotropic solution, and RNA is purified on a glass fiber filter
cartridge specifically designed for very small samples. A simple modification
of the protocol allows recovery of both large and small RNA species including
tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µl.
Residual genomic DNA is efficiently removed using the DNA-free system included in the kit. RNAqueous-Micro contains enough
reagents for 50 RNA isolations of DNA-free RNA.