EGGstract® IgY Purification System
from
Promega
Description
The EGGstract® IgY Purification System provides an easy and rapid method for the isolation of immunoglobulins (IgY antibodies) from egg yolks. Because a single egg contains as much antibody as an average bleed from a rabbit, this represents an appealing alternative to the generation of pAb in rabbits. Additionally, IgY can be produced in chickens against certain biological antigens that fail to elicit a humoral response in rabbits or other mammals due to species relatedness. The procedure is very simple and requires less than one hour to complete. To use the system in purification of IgY antibodies from eggs, simply separate the yolks from immunized chicken eggs and dilute the yolks into Precipitation Solution A. After mixing, remove the lipids by centrifugation and precipitate the IgY using Precipitation Solution B. The solution is centrifuged, and the pellet obtained from solution contains the IgY antibody.