AminoLink Plus Coupling Gel
from
Thermo Scientific Pierce Protein Research Products
Description
AminoLink® Plus Coupling Gel allows simple and efficient covalent immobilization of a wide variety of proteins to a solid support providing a valuable tool for purifying an antibody, antigen or other biomolecule. This activated support contains aldehyde functional groups that react with primary amines present on proteins or other molecules. Reductive amination of the resulting Schiff bases produces a stable secondary amine linkage with minimal ligand leakage. Coupling efficiency is typically greater than 80%, regardless of the ligand’s molecular weight or pI. Additionally, this gel is composed of a beadedagarose matrix with fast linear flow potential. The support, therefore, can be used for low- to medium-pressure applications.
Two coupling protocols (pH 7.2 and pH 10) are offered for use with AminoLink® Plus Coupling Gel. The pH 10 coupling protocol typically provides greater immobilization yields and ligand densities than the pH 7.2 protocol. The standard, 7.2 pH protocol is offered as an alternative for proteins that are sensitive to the pH 10 environment required for the enhanced coupling protocol.