QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
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EMD Millipore
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Product
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QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Applications
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Activity Assay
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Detection
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Fluorescent
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Reactivity
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Human
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Source/Expression System
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Cytology test kits or supplies
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Target
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Cell Invasion
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Catalog Number
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ECM555
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Method
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Fluorometric (CyQuant)
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Original Item Name
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QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
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Quantity
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1 plate
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Sample Type
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Cells
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Target/Molecule Descriptor
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QCM™
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Description
QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric Cell Migration / Invasion. Invasion through the extracellular matrix (ECM) is an important step in tumor metastasis. Cancer cells initiate invasion by adhering to and spreading along the blood vessel wall. Proteolytic enzymes, such as MMP collagenases, dissolve tiny holes in the sheath-like covering (basement membrane) surrounding the blood vessels to allow cancer cells to invade (Egeblad & Werb, 2002). Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. However, current methods of analysis are time-consuming and tedious, involving cotton swabbing of non-invaded cells on the topside of insert, manual staining and counting. Recently a fluorescence blocking membrane insert was introduced to address these issues; however, this approach requires labeling of the cells with Calcein-AM and extensive washing to remove free Calcein before cell invasion. The effect of this treatment on cell behavior/invasion remains questionable. The CHEMICON QCM™ 96-well Invasion Assay does not require cell labeling, scraping, washing or counting. The 96-well insert and homogenous fluorescence detection format allows for large-scale screening and quantitative comparison of multiple samples. In the CHEMICON QCM™ 96-well Invasion Assay, invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer. These cells are subsequently lysed and detected by the patented CyQuant GR* dye (Molecular Probes) (Gildea et al., 2000). This green-fluorescent dye exhibits strong fluorescence enhancement when bound to cellular nucleic acids (Jones et al., 2001). *CyQUANT GR® is a registered trademark of Molecular Probes, Inc. The reagent is licensed from Molecular Probes, Inc. and is for use in kits sold by Chemicon International, Inc. for the monitoring of cell invasion and cell migration only. The CHEMICON Cell Invasion Assay Kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model. The kit utilizes ECMatrixTM, a reconstituted basement membrane matrix of proteins derived from the Engelbreth Holm-Swarm (EHS) mouse tumor (Albini et al., 1987; Repesh, 1989; Terranova et al., 1986; Liotta, 1984). We examined the kits performance using human fibrosarcoma (HT-1080) and non-invasive fibroblasts (NIH3T3). The CHEMICON QCM™ 96-well Invasion Assay provides a quick and efficient system for quantitative determination of various factors on cell invasion, including screening of pharmacological agents, evaluation of MMPs or other proteases responsible for cell invasion, or analysis of gene function in transfected cells. In addition, CHEMICON also provides QCM™ 96-well Chemotaxis Cell Migration Assay Systems (8 µm or 3 µm pore), 24-well insert Cell Migration and Invasion Assay Systems, CytoMatrix™ Cell Adhesion strips coated with ECM proteins or anti-integrin antibodies, and QuantiMatrix™ ECM protein ELISA kits. Test Principle: The CHEMICON Cell Invasion Assay is performed in a 96 well invasion plate based on the Boyden chamber principle. This plate contains 96 inserts; each insert contains an 8 µm pore size polycarbonate membrane coated with a thin layer of ECMatrixTM. The ECM layer occludes the membrane pores, blocking non-invasive cells from migrating through. Invasive cells, on the other hand, migrate through the ECM layer and cling to the bottom of the polycarbonate membrane. Invaded cells on the bottom of the insert membrane are dissociated from the membrane when incubated with Cell Detachment Buffer and subsequently lysed and detected by CyQuant GR dye. Application: The CHEMICON Cell Invasion Assay Kit is ideal for evaluation of invasive tumor cells. Each CHEMICON Cell In
Specifications/Features
Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.;96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.;Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.;4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.;CyQuant GR Dye®: (Part No. 90132) One vial - 75 µL