pGeneBLAzer™ TOPO® TA Expression Kit for in vivo detection
from
Invitrogen
Description
The pGeneBLAzer® TOPO® TA Expression Kits allow five-minute cloning of Taq polymerase-amplified PCR products into a vector for promoter function analysis in mammalian cells using the β-lactamase reporter gene. Promoter sequences are cloned upstream of the β-lactamase reporter gene. Recombinant vectors can then be transfected into mammalian cells and assayed for promoter function and strength in vivo or in vitro using the GeneBLAzer® In Vivo or In Vitro Detection Kit, respectively. No ligase, post-PCR procedures, or PCR primers containing specific sequences are required.
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert, especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and / or cause the production of non-functional protein.
In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions.
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO® cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.