pLenti6/V5 Directional TOPO Cloning Kit
from
Invitrogen
|
|
|
|
Product
|
pLenti6/V5 Directional TOPO Cloning Kit
|
|
Company
|
Invitrogen
|
|
Price
|
Pricing Info
|
|
More Information
|
View Company Product Page
|
|
Applications
|
Mammalian Expression and Cloning
|
|
Host
|
Mammalian
|
|
Origin of Replication
|
pUC
|
|
Promoter
|
CMV
|
|
Quantity
|
20 reactions
|
|
Resistance
|
ampicillin/ blasticidin
|
|
Type
|
Plasmid
|
|
Vector Name
|
pLenti6/V5-D-TOPO, pLenti6/V5-GW/lacZ
|
|
Catalog Number
|
K495510
|
|
Selectable Marker
|
Blasticidin
|
|
|
|
Description
The pLenti6 / V5 Directional TOPO® Cloning Kit contains the TOPO®-adapted ViraPower™ lentiviral expression vector, pLenti6 / V5-D-TOPO® for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving high-level, constitutive expression of the target gene and the blasticidin selection marker for stable selection in mammalian cells.
Advantages
• High efficiency and rapid cloning
• Constitutive gene expression in dividing and non-dividing mammalian cells in vitro or in vivo
• Produces replication-incompetent virus for enhanced biosafety of the system
Key Features
• Directional TOPO® Cloning site for rapid and efficient directional cloning of blunt-end PCR products
• Rouse Sarcoma Virus (RSV) enhancer / promoter for Tat-independent production of viral mRNA in the producer cell line
• Modified HIV-1 5’ and 3’ Long Terminal Repeats (LTR) for viral packaging and reverse transcription
• HIV-1 psi (ψ) packaging sequence for viral packaging
• HIV Rev response element (RRE) for Rev-dependent nuclear export of unspliced viral mRNA
• (CMV) immediate early promoter for high-level constitutive expression of the gene of interest in mammalian cells
• C-terminal V5 epitope for detection of the recombinant protein
• Blasticidin (bsd) resistance gene for selection in E. coli and mammalian cells
• Ampicillin resistance gene for selection in E. coli
• pUC origin for high-copy replication and maintenance of the plasmid in E. coli
Kit includes
pLenti6 / V5-D- TOPO® Reagents
One Shot® Stbl3™ Chemically Competent E. coli
For research use only. Not intended for any therapeutic or diagnostic use.