pEF6/V5-His TOPO TA Expression Kit
from
Invitrogen
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Product
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pEF6/V5-His TOPO TA Expression Kit
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Mammalian Expression and Cloning
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Conjugate/Tag/Label
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V5 epitope tag and polyhistidine region.
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Domain/Region/Terminus
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C-terminal
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Host
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Mammalian
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Promoter
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EF-1α
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Quantity
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20 reactions
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Resistance
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ampicillin/ blasticidin
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Type
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Plasmid
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Vector Name
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pEF6/V5-His-TOPO, pEF6/V5-His-TOPO /LacZ
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Catalog Number
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K961020
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Selectable Marker
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Blasticidin
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Description
The pEF6/V5-His TOPO® TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO¤ Cloning. The pEF6/V5-His-TOPO® vector includes:
- Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells
- The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
- A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
- A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)