pcDNA™3.1 Directional TOPO Expression Kit
from
Invitrogen
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Product
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pcDNA™3.1 Directional TOPO Expression Kit
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Mammalian Expression and TA Cloning
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Domain/Region/Terminus
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C-terminal
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Host
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Mammalian
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Promoter
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CMV
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Quantity
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40 reactions
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Resistance
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ampicillin/ neomycin
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Type
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Plasmid
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Vector Name
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pcDNA3.1D/V5-His /LacZ, pcDNA3.1D/V5-His-TOPO
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Catalog Number
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K490040
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Selectable Marker
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Geneticin® (G-418)
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Description
The pcDNA™3.1 Directional TOPO® Expression Kit uses linearized, topoisomerase I-activated pcDNA3.1D⁄V5-His-TOPO® for five-minute directional cloning and subsequent high-level expression. Directional Cloning technology facilitates expression experiments because:
- A proofreading enzyme is used for fewer errors in cloned genes
- Greater than 90% of the clones are in the correct orientation for expression, reducing time spent colony screening for clone orientation
In addition, pcDNA3.1D⁄V5-His-TOPO® provides strong expression levels from the CMV promoter and the option of a C-terminal V5-His fusion tag for easy detection of recombinant protein with an Anti-V5 Antibody and rapid purification on nickel-chelating resin.
Specifications/Features
Kit Contents:
Each pcDNA™3.1 Directional TOPO¤ Expression Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot® box contains single-use 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. Guaranteed stable for 6 months when properly stored.