pEF5/FRT/V5 Directional TOPO Cloning Kit
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Invitrogen
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Product
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pEF5/FRT/V5 Directional TOPO Cloning Kit
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Mammalian Expression and Cloning
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Conjugate/Tag/Label
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V5 epitope
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Domain/Region/Terminus
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C-terminal
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Host
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Mammalian
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Promoter
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EF-1α
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Quantity
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20 reactions
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Resistance
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ampicillin/ hygromycin
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Type
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Plasmid
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Vector Name
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pEF5/FRT/V5/GW-CAT, pEF5/FRT/V5-D-TOPO
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Catalog Number
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K603501
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Selectable Marker
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Hygromycin
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Description
Using restriction enzymes to clone your gene into an expression vector often forces you to compromise the final sequence of your insert , especially when there are no useful restriction sites close to your genes coding sequence. This may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, which can reduce your expression levels and/or cause the production of non-functional protein.
In addition to being a more effective way to clone, TOPO® Cloning eliminates these potential expression problems. TOPO® Expression Vectors enable you to insert the exact DNA sequence you require simply by performing PCR with appropriately designed primers. Your PCR product is cloned at a high efficiency in only five minutes into a topoisomerase I-activated expression vector. The resulting recombinant expression vector contains your exact DNA sequence without any non-coding regions (Figure 1B).
Many of Invitrogen's powerful expression vectors are available adapted for one-step TOPO® cloning and expression of PCR products. In addition, several expression vectors are now adapted for Directional TOPO® Cloning, enabling you to use proofreading polymerases and to clone your PCR products in a specific orientation.
Specifications/Features
Vector Size:
5831 bp