pcDNA™3.2-DEST
from
Invitrogen
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Product
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pcDNA™3.2-DEST
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Mammalian Expression and Cloning
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Conjugate/Tag/Label
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V5 epitope
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Domain/Region/Terminus
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C-terminal
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Host
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Mammalian
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Promoter
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CMV
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Quantity
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6 µg
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Resistance
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Chloramphenicol / neomycin / blasticidin
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Type
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Plasmid
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Vector Name
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pcDNA3.2/V5/GW-CAT, pcDNA3.2/V5/GW/CAT, pcDNA3.2/V5-DEST
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Catalog Number
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12489019
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Selectable Marker
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Geneticin® (G-418)
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Description
The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The pcDNA3.2/V5-DEST vector offers the following key features:
•Cytomegalovirus (CMV) promoter for high-level expression
•attR sites for Gateway® cloning, enabling recombination with attL-flanked fragments
•C-terminal V5 tag for easy detection
•Neomycin resistance gene for stable selection
•Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway® Cloning
To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway® entry clone, appropriate Gateway® LR Clonase® enzyme mix, and reaction buffer.
Specifications/Features
Vector Size:
7.7 kb
Kit Contents:
Gateway® Destination Vector, Control Plasmid