Zero Background™/Kan Cloning Kit
from
Invitrogen
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Product
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Zero Background™/Kan Cloning Kit
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Company
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Invitrogen
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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End Specific Cloning
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Host
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Bacteria
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MCS
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Nsi I* Hind III Asp718 I Kpn I Ecl136 II, Sac I, BamH I/Bst I, Spe I, EcoR I, Pst I, EcoR V, Not I, Xho I, Nsi I*, Xba I, Dra II, Apa I
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Origin of Replication
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pUC, F1 ori
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Promoter
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T7 and SP6
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Resistance
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kanamycin
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Type
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Plasmid
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Vector Name
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pZErO-2
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Catalog Number
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K260001
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Quantity
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25 µg
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Selectable Marker
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LacZ
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Description
The Zero Background™ Cloning Kits include pZErO® vector with Zeocin™ or kanamycin resistance for selection in E. coli. The pZErO® vectors use positive selection to eliminate high background. There are two vectors to choose from:
- the pZErO®-1 vector takes advantage of the small size of the Zeocin™ resistance gene to reduce the overall vector size for increased ligation and transformation efficiencies
- the pZErO®-2 vector includes the kanamycin resistance gene
Specifications/Features
Kit Contents:
Each kit includes 25 µg supercoiled pZErO®-1 (the Zero Background Cloning Kit)or pZErO®-2 vector (the Zero Background™/Kan Cloning Kit) in TE, 200 ng blunt-end test inserts, TOP10 E. coli stab, 25 µl T4 DNA ligase, 10X ligation buffer, 1 ml sterile water, and 2 ml sterile TE. The Zero Background™ Cloning Kit also contains 1.25 ml Zeocin™ selection agent. Store the TOP10 stab at room temperature. Store the remaining kit components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Features:
Blunt Cloning Kits