T7 RNA Polymerase
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Affymetrix / USB
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Product
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T7 RNA Polymerase
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Company
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Affymetrix / USB
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Production of RNA transcripts for hybridization probes. Production of large amounts of discrete sized RNA. Production of capped or modified RNA transcripts.
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Original Item Name
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T7 RNA Polymerase
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Source/Expression System
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E. coli strain containing an overproducing clone of T3 RNA Polymerase.
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Catalog Number
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70001Z 30 KU
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Quantity
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30 ku
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Concentration
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Standard Concentration (PN 70047Y): 20 units/µl
High Concentration (PN 70001Y/Z): >80 units/µl
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Description
Description:
T7 RNA Polymerase is a single-subunit enzyme produced by bacteriophage T7(2). It is highly specific for T7 promoter and terminator sequences(2,3). It has been widely used for the rapid synthesis in vitro of specific RNAs. These transcripts can be used directly as substrates for studies of RNA structure or metabolism. If the transcripts are suitably labeled, they can also be used as sensitive hybridization probes. T7 RNA Polymerase, along with chain-terminating nucleoside trihosphates(4), have also been used for the direct sequencing of DNA. T7 RNA Polymerase is also used to generate capped mRNA for expression studies in oocytes and other cells(5).
Specifications/Features
Unit Definition:
One unit is the amount of enzyme required to catalyze the incorporation of 1nmol of labeled nucleoside triphosphate into acid insoluble material in 60 min at 37°C, under standard assay conditions.