Exonuclease-Free Klenow
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Affymetrix / USB
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Product
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Exonuclease-Free Klenow
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Company
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Affymetrix / USB
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Random primed DNA labeling.Strand displacement amplification (SDA)(3).DNA sequencing by the Sanger dideoxy method(2).Labeling recessed 3 termini of DNA fragments with labeled dNTP.Note: Exonuclease-Free Klenow is not recommended for fill-in reactions before DNA ligation, since it frequently adds one or more extra nucleotides to the 3-terminus of a blunt-ended DNA substrate in a non-template directed fashion(4).
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Original Item Name
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Exonuclease-Free Klenow
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Source/Expression System
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E. coli strain containing an overproducing clone of E. coli Exonuclease I.
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Catalog Number
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70057Z 750 UN
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Quantity
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750 units
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Concentration
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10 U/µl
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Description
Description:
The large fragment of DNA polymerase I from E. coli, Klenow, has been genetically engineered to eliminate the 3→5 exonuclease activity normally associated with this polymerase(1). Exo-free Klenow is useful in applications where accompanying exonuclease activity is of significant concern, such as random primed labeling, DNA sequencing by the Sanger, et al. method, or strand displacement amplification (SDA).
Specifications/Features
Comments:
70057Z 750 UN
Unit Definition:
One unit is the amount of enzyme required to catalyze the incorporation of 10nmol of total deoxyribonucleotides into an acid-precipitable material in 30 min at 37°C using activated DNA as a template-primer