T4 Polynucleotide Kinase (PNK)
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Affymetrix / USB
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Product
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T4 Polynucleotide Kinase (PNK)
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Company
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Affymetrix / USB
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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5-labeling of DNA and RNA. Mapping termini of RNA transcripts. Phosphorylation of oligonucleotides at 5 termini prior to ligation. Removal of 3-phosphoryl groups.
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Original Item Name
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T4 Polynucleotide Kinase (PNK)
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Source/Expression System
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E. coli strain containing a modified clone of T4 Polynucleotide Kinase that has full kinase activity but lacks phosphatase activity.
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Catalog Number
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70031Z 1000 UN
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Quantity
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1000 units
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Concentration
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30 units/µl
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Description
Description:
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the terminal phosphate of ATP to
5 hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3 mononucleotides(2). In the presence of ADP, it will also catalyze the exchange of 5 terminal phosphate groups and ATP. It also possesses a 3 phosphatase(4,5,8,9) and 2,3 cyclic phosphodiesterase activity(3). This enzyme is commonly used to label DNA or RNA with 32P or 33P at 5 ends(6).
Specifications/Features
Unit Definition:
One unit is the amount of enzyme required to incorporate one 1nmol of radiolabeled ATP into micrococcal nuclease-treated DNA in 30 min at 37°C