Polynucleotide Kinase, T4
from
Affymetrix / USB
Description
Description:
T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the terminal phosphate of ATP to
5' hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3' mononucleotides(2). In the presence of ADP, it will also catalyze the exchange of 5' terminal phosphate groups and ATP. It also possesses a 3' phosphatase(4,5,8,9) and 2',3' cyclic phosphodiesterase activity(3). This enzyme is commonly used to label DNA or RNA with 32P or 33P at 5' ends(6).
Specifications/Features
Unit Definition:
One unit is the amount of enzyme required to incorporate one 1nmol of radiolabeled ATP into micrococcal nuclease-treated DNA in 30 min at 37°C