Exonuclease III
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Affymetrix / USB
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Product
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Exonuclease III
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Company
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Affymetrix / USB
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Price
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Pricing Info
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More Information
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View Company Product Page
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Applications
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Preparing strand-specific radioactive probes with a DNA polymerase.Preparing single-stranded DNA templates for dideoxy sequencing(3). Constructing unidirectional deletions.Long PCR.DNA footprinting.DNA repair/mutation detection studies.
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Original Item Name
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Exonuclease III
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Quantity
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5000 units
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Source/Expression System
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E. coli strain containing an overproducing clone of the E. coli xthA gene (Exonuclease III).
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Catalog Number
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70023Y 5000 UN
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Concentration
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200 units/µl
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Description
Description:
Exonuclease III, the major apurinic/apyramadinic (AP) endonuclease in E. coli(1), is a 3’-5’ exonuclease digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex(2). Exonuclease III breaks phosphodiester bonds on the 5’ side of AP sites in both dsDNA and ssDNA(3), removes 3’ terminal groups on dsDNA(4), increases MutY turnover(5), and efficiently degrades 3’ recessed but not 3’ protruding DNA ends (creating 5’ overhangs)(6). These properties have been exploited to allow strand specific labeling, unidirectional deletions, and selective strand degradation.
Specifications/Features
Unit Definition:
One unit will catalyze the release of 1nmol of nucleotides to acid soluble form in 30 min at 37°C under standard assay conditions