SelectFX® Alexa Fluor® 488 Endoplasmic Reticulum Labeling Kit *for fixed cells*
from
Molecular Probes (Invitrogen)
Description
The SelectFX® Alexa Fluor® 488 Endoplasmic Reticulum Labeling Kit provides all the reagents needed to label the ER in fixed cells. To detect the ER, the kit uses a primary antibody directed against the ER-associated protein disulfide isomerase (PDI) and an Alexa Fluor® 488 dye-labeled secondary antibody. The fluorescence can be observed using the same optical filters used for fluorescein. The kit also includes cell fixative and permeabilization reagents and protocols for mammalian cell preparation and staining.