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MCS
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Bgl II*, Sca I*, Not I*, Nhe I*, EcoR V, Pvu I, Spe I*, Sal I ,Xho I *The presence of stop codons in-frame with the 3× HA tag must be considered when inserting genes into the MCS. Do not use the Bgl II, Not I, Sca I, Nhe I or Spe I sites for cloning unless the cloning strategy removes the stop codons by double digestion using one of the upstream sites plus a site downstream of the stop codons.
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