Cell Adhesion Fluorometric (calcein-AM) Assay Kit, InnoCyte™
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EMD Millipore
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Product
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Cell Adhesion Fluorometric (calcein-AM) Assay Kit, InnoCyte™
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Detection
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Request Info
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Form
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96-well plate
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Target
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Cell Adhesion
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Catalog Number
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CBA026-1KIT
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Method
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Fluorometric
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Original Item Name
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InnoCyte™ ECM Cell Adhesion Assay, Galectin-1/Galectin-3
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Quantity
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72 Tests (36 Gal-1 and 36 Gal-3)
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Sample Type
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Adherent cultured cells
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Target/Molecule Descriptor
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InnoCyte™
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Time
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2.5 h
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Description
PROTECT FROM LIGHT. AVOID FREEZE/THAW CYCLES. The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with galectin-1 and galectin-3. Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader. Includes galectin-1-coated 96-well plate strips, galectin-3-coated 96-well plate strips, calcein-AM solution, D-PBS, and user protocol.
Specifications/Features
Kit Contents:
The InnoCyte™ Cell Adhesion Assay, Galectin-1/Galectin-3 is designed for the determination of the relative attachment of adherent cell lines to galectin-1 and galectin-3, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with galectin-1 and galectin-3. Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.