Crystallization Kit for DNA
from
Sigma-Aldrich
Description
The Crystallization Kit for DNA is an empirical screening method for the direct determination of suitable crystallization conditions for nucleic acids. The kit is developed according to the described method of Nowakowski et al.1, Baeyens, Jancarik and Holbrook2 These ready-made solutions represent a screen of conditions, from which many were observed to produce crystals from DNA oligomers and transfer RNA. This screen provides high efficiency in crystallization of RNA oligomers. Thus it provides a completion of our crystallization kit for DNA.The Crystallization Kit for DNA consists of 48 different formulations covering a range of different buffers (pH 6.0-8.0), different types of precipitants (esp. PEG 400 and MPD), and inorganic salts including magnesium salts. All solutions are sterile filtered using 0.22 micron filters. The kit contains 10 ml of each formulation, but all solutions are available separately as 100 ml bottles. Larger quantities are available on request.
Specifications/Features
. Comprehensive, efficient screening. Extremely pure, sterile reagents. Prefabricated reagents and observation sheets. Low incidence of undesirable solubility effects