Director™ Universal PCR System
from
Sigma-Aldrich
Description
Application: The simple three step procedure (PCR, Exonuclease III digestion and rapid ligation/transformation) can be completed in one day. The typical cloning efficiency using this method is greater than 80%.
Features and Benefits:
- Universal - PCR amplicon can be cloned into any expression vector
- High Cloning Efficiency - Typically>80%
- High Expression Efficiency - Typically>66%
- High Fidelity - Long and accurate, hotstart enzyme generates amplicons up to 20 kb with fidelity up to 6.5Xgreater than standardTaqDNA polymerase
- Fast - Simple three-step procedure allows completion in less than one day