Cell Adhesion Fluorometric (calcein-AM) Assay Kit, InnoCyte™
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EMD Millipore
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Product
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Cell Adhesion Fluorometric (calcein-AM) Assay Kit, InnoCyte™
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Company
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EMD Millipore
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Price
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More Information
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View Company Product Page
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Detection
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Request Info
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Form
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96-well plate
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Target
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Cell Adhesion
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Catalog Number
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CBA025-1KIT
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Method
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Fluorometric
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Original Item Name
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InnoCyte™ ECM Cell Adhesion Assay, Laminin/Basement Membrane Complex (BMC)
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Quantity
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72 Tests (36 Laminin and 36 BMC)
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Sample Type
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Adherent cultured cells
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Target/Molecule Descriptor
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InnoCyte™
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Time
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2.5 h
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Description
PROTECT FROM LIGHT. AVOID FREEZE/THAW CYCLES. The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader. Includes laminin-coated 96-well plate strips, BMC-coated 96-well plate strips, calcein-AM solution, D-PBS, and user protocol.
References
Caroll, D.K., et al. 2006. Nat. Cell Biol. 8, 551.Ekblom, P., et al. 2003. Matrix Biology 22, 35.Li, S., et al. 2002. J. Cell Biol. 157, 1279.Hynes, R.O., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 2588.Aumailley, M., et al. 1998. J. Anat. 193, 1.Nelson, P.R., et al. 1997. Vasc. Surg. 26, 104.Horowitz, A.F., et al. 1996. Trends Cell Biol. 6, 460.Baron von Evercooren, A., et al. 1982. J. Neurosci. Res. 8, 179.
Specifications/Features
Kit Contents:
The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.