SlowFade® Antifade Kit
from
Molecular Probes (Invitrogen)
Description
The SlowFade® Antifade Kit contains two formulations of the SlowFade® Antifade Reagent: an aqueous formulation in buffer and an aqueous formulation that fcontains 50% glycerol. Both formulations reduce the photobleaching rate of fluorescein to almost zero and are especially useful for quantitative measurements. They are ideal for confocal laser-scanning microscopy, in which the excitation intensities can be extreme and prolonged. Use of the SlowFade® Antifade Kit can extend the useful fluorescence emission of fluorescein more than 50-fold and can preserve the signal in cell and tissue mounts for up to two years. This original SlowFade® formulation substantially quenches the fluorescence of fluorescein and almost completely quenches that of the Alexa Fluor® 350, Alexa Fluor® 405, and Cascade Blue® fluorophores. A new formulation, SlowFade Gold is now available with (S36938, S36939) and without DAPI (S36936, S36967).