Description
Unlike many other qRT-PCR kits, MessageSensor includes a total RNA control, a control human GAPDH primer set, RNase inhibitor, and nucleotides, as well as a buffer additive that enables detection with SYBR® Green dye. The MessageSensor Kit is provided with enough reagents for 50 reactions. When purchased in combination with Ambion’s SuperTaq™ Real-Time, the MessageSensor RT Kit provides all the components you need for one-step qRT-PCR (except for gene-specific primers and fluorogenic probe or dye) in a single convenient order.
A detailed instruction manual with separate protocols for performing one-step RT-PCR using TaqMan® probes, SYBR® Green and end-point detection is provided with the kit.
Comments
• More sensitive than SuperScript™ II and other RNase H– MMLV reverse transcriptases
• Linear quantitation of mRNA targets over 106-fold range of input RNA
• Quantitate transcripts from as little as 500 fg total RNA – 1/20th of a single cell!
• Fast, flexible RT step complete in 15 min; Use reaction temperatures up to 50ºC
• Compatible with TaqMan®, SYBR® Green, and gel-based detection methods