MessageSensor™ RT Kit
from
Ambion
Description
For the reverse transcription of RNA. The Ambion® kit includes a M-MLV RT that maintains full RNase H activity for increased sensitivity of detection in RT-PCR experiments. Includes sufficient reagents for 50 reactions.
• Linear quantitation of mRNA targets over 106-fold range of input RNA
• Quantitate transcripts from as little as 500 fg total RNA—1â„20th of a single cell
• Fast, flexible RT step complete in 15 min; use reaction temperatures up to 50°C
• Compatible with TaqMan®, SYBR® Green, and gel-based detection methods
MessageSensor™ is designed to provide unsurpassed results in the least amount of time. The kit contains a unique formulation of M-MLV RT developed for a single tube approach increasing lower-end reaction sensitivity (see figure). The reverse transcription step takes only 15 minutes, so you can go from RNA sample to PCR results in less than 2 hours.
Specifications/Features
Kit Contents:
Unlike many other qRT-PCR kits, MessageSensor includes a total RNA control, a control human GAPDH primer set, RNase inhibitor, and nucleotides, as well as a buffer additive that enables detection with SYBR® Green dye. The MessageSensor Kit is provided with enough reagents for 50 reactions. When purchased in combination with Ambion’s SuperTaq™ Real-Time, the MessageSensor RT Kit provides all the components you need for one-step qRT-PCR (except for gene-specific primers and fluorogenic probe or dye) in a single convenient order.
Comments:
• More sensitive than SuperScript™ II and other RNase H– MMLV reverse transcriptases
• Linear quantitation of mRNA targets over 106-fold range of input RNA
• Quantitate transcripts from as little as 500 fg total RNA – 1/20th of a single cell!
• Fast, flexible RT step complete in 15 min; Use reaction temperatures up to 50ºC
• Compatible with TaqMan®, SYBR® Green, and gel-based detection methods