Cascade Blue® acetyl azide, trisodium salt
from
Molecular Probes (Invitrogen)
Description
The amine-reactive Cascade Blue® acetyl azide and its conjugates yield blue-fluorescence (approximate excitation/emission maxima ~396/410 nm) that can be used as a contrasting color in multicolor applications. When compared with aminocoumarin derivatives, the sulfonated pyrene Cascade Blue® dye shows less spectral overlap with fluorescein. In addition, Cascade Blue® dye exhibits high absorptivity, is highly fluorescent and, unlike most dyes, resists quenching upon protein conjugation.