cdc2 Protein Kinase Radioactive assay Assay Kit, SignaTECT®
from
Promega
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Product
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cdc2 Protein Kinase Radioactive assay Assay Kit, SignaTECT®
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Company
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Promega
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Price
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More Information
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Detection
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Radioactive quantitation of membrane bound biotin conjugated substrates
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Target
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cdc2 Protein Kinase
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Catalog Number
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V6430
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Method
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Radioactive assay
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Original Item Name
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SignaTECT® cdc2 Protein Kinase Assay System
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Quantity
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96 reactions
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Target/Molecule Descriptor
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SignaTECT®
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Description
The SignaTECT® Protein Kinase Assay Systems contain the proprietary SAM2® Biotin Capture Membrane, which offers significant advantages over other radioactive technologies for assaying protein kinases. The streptavidin-coated SAM2® Membranes possess high binding capacity and high specificity characteristics, which produce lower backgrounds and higher signal-to-noise ratios compared to the traditional P81 phosphocellulose method of capture and measurement. The perforated and numbered membrane allows researchers to measure from 1 up to 96 kinase reactions. The SAM2® Membrane format does not require as much "hands-on" manipulation as other methods used to measure kinase activity. Following the kinase reaction, samples are spotted onto the SAM2® Membrane, and a series of short wash steps are performed to remove nonspecific label. The process is complete in less than 1 hour. In addition, the nature of the SAM2® Membrane allows it to be used under a variety of buffer/reaction conditions (e.g., cell extracts), which many other methods do not allow. Lastly, the high binding capacity allows use of the SignaTECT® Systems for kinetic studies. • Each system contains highly specific biotinylated peptide substrates for the appropriate kinase as well as the necessary reaction components. The researcher must supply [γ-32P]ATP.