ProtoBlot® II AP System with Stabilized Substrate, Human
from
Promega
Description
The ProtoBlot® II AP Systems with Stabilized Substrate are designed for the rapid and sensitive detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis or bound directly from solution ("dot" blots; 3). The Western Express® Fast Blotting Protocol is included with the system and can dramatically reduce the time required to perform immunodetection.
Features:
Easy to Use Western Express® Protocol allows the detection of dot blots in 30–45 minutes and the detection of Western blots in 1–2 hours. • The system contains Western Blue® Stabilized Substrate for AP, which is a ready-to-use solution of BCIP/NBT. • No reagent preparation is required for the substrate. • For many applications, AP conjugates are superior to HRP conjugates because they: offer greater sensitivity (tenfold) of detection, are not inhibited by azide, use a substrate that is not prone to fading during long-term storage, have protocols provided for both PVDF and nitrocellulose membranes.