Access RT-PCR System from Promega

Product Specs
ItemAccess RT-PCR System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1280
Quantity500 reactions

Specifications/Features

Kit Contents:
1 x 500u AMV Reverse Transcriptase, 1 x 500u Tfl DNA Polymerase, 1 x 1ml AMV/Tfl 5X Reaction Buffer, 1 x 1250µl MgSO4, 2 x 50µl dNTP Mix, 1 x 50µl Positive Control RNA with Carrier, 1 x 100µl Upstream Control Primer, 1 x 100µl Downstream Control Primer, 1 x 13ml Nuclease-Free Water, 1 x 100 reactions Access RT-PCR System, 4 x 1 each Access RT-PCR Core Reagents

Comments:
A1280

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) En masse evaluation of RNA guides (EMERGe) for ADARsMethods in EnzymologyJanuary 27, 2025Prince J. Salvador, Natalie M. Dugan, Randall Ouye, Peter A. BealIllumina sequencing The post-deamination RNA is concentrated to 350 μL in vacuo and subjected to RT-PCR (Promega A1280). The AMV reverse transcriptase step is performed for 45 min at 45 °C and the resultant cDNA is amplified

(2) Library Screening Reveals Sequence Motifs That Enable ADAR2 Editing at Recalcitrant SitesACS Chemical BiologyOctober 20, 2023Casey S. Jacobsen, Prince Salvador, John F. Yung, Sabrina Kragness, Herra G. Mendoza, Gail Mandel, Peter A. Bealconcentrated in vacuo to a total volume of 350 μL. The concentrated RNA was used for RT-PCR (Promega A1280) to a total volume of 500 μL to generate cDNA. The cDNA was then purified by gel extraction using 2%

(3) Rational Design of RNA Editing Guide Strands: Cytidine Analogs at the Orphan PositionJournal of the American Chemical SocietyMay 12, 2021Erin E. Doherty, Xander E. Wilcox, Lenka van Sint Fiet, Cherie Kemmel, Janne J. Turunen, Bart Klein, Dean J. Tantillo, Andrew J. Fisher, Peter A. BealRQ1 RNase-free DNase (Promega, M6101). Nested RT-PCR was performed in triplicate using Access RT-PCR kit (Promega, A1280) for 20 cycles and then followed by Phusion Hot Start II DNA Polymerase (Thermo Fisher, F-549L) for

(4) Markers of Pluripotency in Human Amniotic Epithelial Cells and Their Differentiation to Progenitor of Cortical NeuronsPloS oneJanuary 1, 2015García-Castro IL, García-López G, Ávila-González D, Flores-Herrera H, Molina-Hernández A, Portillo W, Ramón-Gallegos E, Díaz NF.(Life Technologies) and was reverse transcribed and amplified using a one-step RT-PCR system (Promega A1280, Madison, WI, USA) and the following forward (F) and reverse (R) primers (Integrated DNA Technologies

(5) Isolation of viral hemorrhagic septicemia virus from Greenland halibut Reinhardtius hippoglossoides caught at the Flemish CapDiseases of Aquatic OrganismsJuly 29, 2002C P Dopazo, I BandÃn, C LÃ³pez-Vazquez, J Lamas, M Noya, J L Barja

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